Today’s episode is about practical tools that we can use to improve our biology and how we can track those results to make sure we are getting the right answers. This episode can serve as an important source of information about N=1 experiments and biohacking.

N=1 experiments involve a single subject and they are entirely capable of providing statistical inferences about the efficacy or side-effects of treatments specifically on that subject alone. The aim of this episode is to provide very practical tips that are really accessible to you. Some of the topics covered are the Bulletproof diet, intermittent fasting, and the impact of oxaloacetate supplements.

“So we could run the same experiment…and your results can be different from mine, but it doesn’t mean that either are wrong, it just means that we’re all individuals. Our results apply to ourselves and we [need to approach it in] a different way in terms of how we want to improve or optimize something.”
– Bob Troia

Bob Troia’s quest for self knowledge, betterment, and optimization inspired his own self-tracking, biohacking, and n=1 experiments. Some of Bob’s experiments have included glucose hacking and tracking, telomere analysis, bulletproof diet (cholesterol/bloodwork), and central nervous system (CNS) training. He has had the opportunity to give several Quantified Self talks on his glucose tracking experiments. 

Bob is also a successful tech entrepreneur, and is currently working on a new venture, HuMend, which is developing a solution to treat musculoskeletal injuries using 3D printing technology. Bob holds a Bachelor of Science degree from Pennsylvania State University in Agricultural and Biological Engineering.

The show notes, biomarkers, and links to the apps, devices and labs and everything else mentioned are below. Enjoy the show and let me know what you think in the comments!

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Show Notes

  • Bob’s interest in quantified self and biohacking results from trying to uncover and understand what makes him tick and how to optimize and improve it. (Time 5:58)
  • N=1 experiments are implemented on one person. They are not scientifically applicable to the whole population. (Time 8:00)
  • One of Bob’s earliest n=1 experiments involved a paleo-like diet called the Bulletproof diet. (Time: 9:00)
  • There are a number of online services that can facilitate your bloodwork testing. (Time 12:55).
  • There is a big difference between traditional and functional medicine. The normal ranges for traditional medicine may not be applicable to individuals based on their unique genetic composition. Services such as InsideTracker and WellnessFX may give you a “range” for your results, but you may need a functional medical practitioner to further investigate the details. (Time 14:00).
  • Part of a low white blood cell count is not that your immune system isn’t kicking up; it’s that it’s being suppressed. (21:00).
  • An underactive thyroid is linked to elevated LDLs. Bob was introduced to some programs that support thyroid and adrenal functions, and that was a shortcut which led to improving numbers such as total cholesterol, LDL, and testosterone. (28:00)
  • Bob’s recommendation is to find a medical practitioner with more of a functional medicine background. (29:50)
  • Another of Bob’s recommendations is to find a medical practitioner who has an investigative mindset.(31:16)
  • Bob sees the philosophy of “Quantified Self” evolving into “Quantified Team.” (33:00)
  • Bob gets testing every three months. He is still investigating having more short-term testing, for instance on a monthly basis. (37:00)
  • The biomarkers Bob tracks on a routine basis range from basic panels, cholesterol markers, glucose, nutrients like calcium, magnesium, vitamin D, white blood cells, C-reactive protein, and an MDL test that can check for chronic infections. (38:00)
  • As part of a longevity strategy and to maintain optimal glucose levels, Bob recommends a supplement called oxaloacetate. (48:00)
  • Other recommendations include the Calm app and binaural beats (Holosync) as tools for meditation. (54:30).
  • Bob’s biggest recommendation is to prevent your data from becoming a hindrance. It is ultimately more about how you feel. People have the tendency to over think it, instead of just starting to do it.  (1:34:20)

Thank Bob Troia on Twitter for this interview.
Click Here to let him know you enjoyed the show!

Bob Troia

  • quantifiedbob.com: Bob Troia’s personal self-tracking, biohacking, n=1 experiments, and Quantified Self tools and resources.
  • Bob on Twitter @QuantifiedBob

The Tracking

Biomarkers

  • Cortisol to DHEA Ratio: Cortisol is a stress hormone and DHEA is a precursor to testosterone and estrogen. Both are produced by the adrenal glands. Since they work in an opposing manner, they are more efficiently measured as a ratio. A normal ratio is approximately 5:1 to 6:1. An abnormal ratio indicates a problem with the adrenal glands.
  • hs-CRP (high sensitivity C-Reactive Protein): A marker of inflammation. The hs-CRP test accurately measures low levels of C-reactive protein to identify low but persistent levels of inflammation which is an indicator for cardiovascular disease (CVD), overtraining and other systemic inflammation issues. In a previous episode (episode 19), Dr. Garry Gordon notes hs-CRP may or may not be a sensitive enough marker of inflammation as it depends on the location and type of inflammation. Also, C-reactive protein is discussed previously in Episode 7 for tracking CVD risk.
  • Fasting glucose: Fasting glucose is one of the clinical markers for blood sugar regulation and can indicate a progression toward diabetes. In order to establish a baseline, Bob performed a fasting glucose measurement with eight hours of fasting before each morning.
  • HRV (Heart Rate Variability): HRV is one biomarker that is a good indicator for overall health and fitness. A high HRV shows that the parasympathetic response is dominant, and vice versa for a low HRV. A high HRV score – greater variability in the time gap between heart beats – is a good thing because it indicates a healthy, fit, well­-rested heart. Damien has found it beneficial to take his HRV readings every morning because a dip could be an indicator of additional stress load. We’ve covered HRV in many episodes (see here)
  • Telomere length: Telomeres are the protective DNA structures at the ends of chromosomes. Over time these protective structures shorten and degrade, as a result of the aging process in general for instance. By measuring telomere length, we’re able to identify how short they are against benchmarks, such as the societal norm, or sub-groups, for a typical age and gender, and use as a proxy for the aging process and how we are faring against it.
  • LDL (Low Density Lipoprotein): The traditional measure of ‘bad cholesterol’ that many doctors still use to evaluate cardiovascular risk, but for which research has now determined not to be the best predictor of cardiovascular risk. However, LDL is also associated with other risks can be a useful marker in general – such as an underactive thyroid as mentioned by Bob in this episode.

Lab Tests, Devices and Apps

  • InsideTracker: InsideTracker is a personalized health analytics company with a platform that tracks and analyzes key biochemical and physiological markers, and applies sophisticated algorithms and large scientific databases to determine personalized optimal zones for each marker.
  • WellnessFX: WellnessFX is a platform that visualizes your blood test results over time, as well as detailed descriptions of each biomarker for an easy interpretation of your overall health. WellnessFX also offers personalized consultations with licensed health practitioners for even more insight into your health.
  • 23andMe genetic testing: A service that provides a DNA kit for collecting samples and analyzing DNA.
  • MDL (Medical Diagnostics Laboratories) testing: A one vial test that can expose different pathogens. Bob referred to this test as one that extends more than the traditional panel and can indicate the presence of chronic diseases.
  • LabCorp: Laboratory Corporation of America provides lab testing and services, with expertise in esoteric testing, genomics, and clinical and anatomic pathology.
  • DirectLabs: DirectLabs offers blood chemistry tests directly to you online at extremely discounted prices with results available in as little as 24 hours for most tests.
  • GeneticGenie: Shows your free methylation and/or detoxification profile after sending a saliva sample to 23andMe genetic testing.
  • Promethease: Compares a person’s DNA data with entries in SNPedia, a public wiki on human genetics. Also can use data imported from 23andMe.
  • TeloMe: A company that offers saliva-based telomere length testing and analysis.

The Tools & Tactics

Diets and Nutrition

  • Intermittent Fasting: Involves consuming most of your calories during a very small window, typically 6 hours and fasting the remainder of the day.
  • Paleo Diet A diet that mimics the nutrition of early hunter-gatherers, and consists of all lean meats and fish, fresh fruits, and nonstarchy vegetables.
  • Bulletproof Diet: A diet that involves skipping breakfast, not counting calories, eating high levels of healthy saturated fat, working out and sleeping less, and adding smart supplements.
  • Holosync: A form of audio technology that is said to induce brain wave patterns such as those of deep meditation.

Supplements

  • Oxaloacetate: Oxaloacetate, the common name for the molecule 3-carboxy-3-oxopropanoic acid and synonymous with oxaloacetic acid (depending on acidity.  is an intermediate of the Kreb’s cycle and the stage immediately prior to the formation of  pyruvate (viapyruvate carboxylase) and immediately after the NAD+-consuming conversion from L-malate (via malate dehydrogenase). It is thought to help with glucose metabolism and reduce variability as well as promote longevity due to being an intermediate of the Kreb’s cycle of energy production.

Tech

  • Muse Calm: A consumer EEG device and app that is designed to help you meditate effectively. Damien refers to his use of this.

Other People, Books & Resources

People

  • Dave Asprey: Dave Asprey, Founder and CEO of bulletproof, was mentioned by the guest as someone whose talks on the principles and logic of the “bulletproof” diet attracted him to the paleo-like diet.
  • Jimmy Moore: Jimmy Moore, a previous guest, is an expert on measuring ketones and optimizing ketogenic diets. Moore also spoke about intermittent fasting during his episode.
  • William J. Walsh: Walsh was a previous guest (episode 2) who is an expert on brain-related disorders. He was mentioned in this episode as helping to do certain labs that help assess micronutrient deficiencies or differences that are out of functional ranges including vitamin B6.
  • Ray Kurzweil: An American author, computer scientist, inventor, and futurist. Author of the books on longevity and extending lifespan Fantastic Voyage: Live Long Enough to Live Forever and Transcend: Nine Steps to Living Well Forever. Ray is mentioned in this episode as one of the proponents of solving a problem before going to sleep.
  • Aubrey de Grey: Chief Science Officer for the SENS Research Foundation, a not-for-profit organization funding research into longevity around the world. Aubrey de Grey is featured in episode 14.
  • Tim Ferriss: An American author, entrepreneur, angel investor, and public speaker. The Four Hour Body, authored by Tim Ferriss, is one of the early books that helped Bob.

Full Interview Transcript

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